Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Silex cells | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1M succinic acid-phosphate-glycine buffer with 25% (w/v) PEG 1500 at pH 4.0 | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
allyl-isothiocyanate | about 30% inhibition at 0.01 mM | Drosophila melanogaster | |
Berberine | complete inhibition at 0.1 mM | Drosophila melanogaster | |
Caffeine | about 25% inhibition at 0.01 mM | Drosophila melanogaster | |
chrysin | about 30% inhibition at 0.1 mM | Drosophila melanogaster | |
coumarin | about 17% inhibition at 0.01 mM | Drosophila melanogaster | |
denatonium benzoate | about 10% inhibition at 0. 1 mM | Drosophila melanogaster | |
Digitonin | about 20% inhibition at 0.01 mM | Drosophila melanogaster | |
escin | about 10% inhibition at 0.1 mM | Drosophila melanogaster | |
fisetin | 60% inhibition at 0.1 mM | Drosophila melanogaster | |
flavonol | about 20% inhibition at 0.01 mM | Drosophila melanogaster | |
kaempferol | about 42% inhibition at 0.01 mM | Drosophila melanogaster | |
luteolin | about 16% inhibition at 0.01 mM | Drosophila melanogaster | |
naringenin | 42% inhibition at 0.1 mM | Drosophila melanogaster | |
naringin | about 17% inhibition at 0.01 mM | Drosophila melanogaster | |
Nicotine | about 16% inhibition at 0.01 mM | Drosophila melanogaster | |
papaverine | about 5% inhibition at 0.1 mM | Drosophila melanogaster | |
paraxanthine | about 9% inhibition at 0.01 mM | Drosophila melanogaster | |
phenethyl-isothiocyanate | about 25% inhibition at 0.01 mM | Drosophila melanogaster | |
piperidine | about 15% inhibition at 0.1 mM | Drosophila melanogaster | |
propyl-isothiocyanate | 42% inhibition at 0.01 mM | Drosophila melanogaster | |
Pyridine | about 17% inhibition at 0.1 mM | Drosophila melanogaster | |
quercetin | complete inhibition at 0.1 mM, 52% inhibition at 0.01 mM | Drosophila melanogaster | |
Quinine | about 18% inhibition at 0.1 mM | Drosophila melanogaster | |
sinigrin hydrate | about 28% inhibition at 0.01 mM | Drosophila melanogaster | |
strychnine | about 37% inhibition at 0.01 mM | Drosophila melanogaster | |
tangeretin | complete inhibition at 0.1 mM | Drosophila melanogaster | |
taurine | about 19% inhibition at 0.1 mM | Drosophila melanogaster | |
Theobromine | about 12% inhibition at 0.01 mM | Drosophila melanogaster | |
theophylline | about 10% inhibition at 0.01 mM | Drosophila melanogaster | |
thiamine hydrochloride | about 5% inhibition at 0.1 mM | Drosophila melanogaster | |
thiazolidine | about 18% inhibition at 0.01 mM | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49850 | - |
SEC-MALS analysis | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VG98 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap column chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
antenna | - |
Drosophila melanogaster | - |
foreleg | - |
Drosophila melanogaster | - |
head | - |
Drosophila melanogaster | - |
maxillary palp | - |
Drosophila melanogaster | - |
olfactory organ | - |
Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
with propyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
36 | - |
with allyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
74 | - |
with phenethyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Drosophila melanogaster | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + allyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? | |
glutathione + phenethyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? | |
glutathione + propyl-isothiocyanate | - |
Drosophila melanogaster | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 25000, SDS-PAGE | Drosophila melanogaster |
homodimer | 2 * 24535, calculated from amino acid sequence | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
GST Delta 2 | isoform | Drosophila melanogaster |
GSTD2 | isoform | Drosophila melanogaster |
Organism | Comment | Expression |
---|---|---|
Drosophila melanogaster | in the olfactory organs, the GSTD2 mRNA level is strongly decreased (5.9fold) in the presence of phenethyl-isothiocyanate | down |
Drosophila melanogaster | isoform GSTD2 expression levels increase in the presence of propyl-, phenethyl- and allyl-isothiocyanates with phenethyl-isothiocyanate (0.25 mM) inducing the highest effect (6fold increase). Phenethyl-isothiocyanate highly induces GSTD2 mRNA expression in the taste organs (3.5fold) | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a biological role in chemoperception | Drosophila melanogaster |